ZFIN Image: Owen et al., 2022, Fig. 4

Image

Figure Caption

Fig. 4 Figure 4. MO knockdown and rescue experiments of bmpr1ba/b in zebrafish. Injection of the MOs alone showed reduction in eye size and the presence of a coloboma. A. Coinjection with in vitro synthesized human BMPR1B messenger RNA (mRNA) rescues the phenotypic effects of the MO alone. Human variants identified within the Genomics England cohort were expressed and mRNAs coinjected with each MO. Scale bar = 500 μm. Eye diameter was reported for (B) bmpr1ba and (C) bmpr1bb compared with wild-type uninjected; MO; MO + human wild-type BMPR1B; and variants Arg91Ile, Arg224His, Arg224Leu, and Arg376Glu. (n for bmpr1ba experiments = 23, 25, 55, 13, 21, 28, 42 and n for bmpr1bb experiments = 23, 56, 55, 32, 34, 33, 24). Unpaired t tests were used to compare data. ∗∗P < .01, ∗∗∗P < .001, ∗∗∗∗P < .0001, ns P > .05. MO, morpholino; ns, nonsignificant.

Figure Data

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Genet. Med.