Image
|
Figure Caption
Fig. 1
Figure 1. Phenotype of VARS2-MO-injected embryos in comparison to control-MO-injected embryos with 73% knockdown efficiency: n = 100 control-MO- and n = 100 splice-MO-injected embryos. Scale bar: 0.5mm (A). Heart rate and ventricular fractional shortening in control-MO- and splice-MO-injected embryos at 48h, 72h, and 96h post-fertilization (n = 25 each) (B). Gel electrophoresis showing a fraction of wild-type cDNA consisting of exons 2, 3, and 4 (241 bp) in control-MO-injected embryos and both wild-type and spliced cDNAs in splice-MO-injected embryos, with spliced cDNA missing exon 3 (159bp) (C). Whole-mount RNA antisense in situ hybridization showing expressions of atrial- and ventricle-specific myosin heavy chains as well as notch1b (D). v: ventricle, a: atrium, hpf: hours post-fertilization. Scale bar: 100µm.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Int. J. Mol. Sci.