Figure 4

Figure 4

Mutation of crlf3 impacts early definitive hematopoiesis. (A–X). Homozygous wild-type crlf3^wt/wt (wt/wt) and crlf3^mdu14/mdu14 (mdu14/mdu14) embryos were subjected to WISH with cmyb(A, B) at 4 dpf, and lcp1(D, E), mpo(G, H), hbbe(J, K), ikzf1(M–N), rag1(P, Q) and tcra(S, T) at 5 dpf (scale bar = 200 μm), or underwent blood analysis (V–W) at 5 dpf (scale bar = 10 μm). Individual embryos were assessed for the area of staining of cmyb CHD region (dotted boxes in panels A and B) (C), hbbe(L), ikzf1(O), rag1(R) and tcra(U), as well as the number of lcp1⁺(F) and mpo⁺(L) cells or for blood differential counts (X), with mean and SEM in red and statistical significance indicated (***p < 0.001, **p < 0.01, *p < 0.05, ns, not significant). Welch’s correction was used for panels (C, L).