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Fig. 1
Zebrafish oocytes store pou5f3 mRNA as RNA granules that are different from those of cyclin B1 mRNA
(A–C) Distributions of pou5f3 mRNA (A and B) and cyclin B1 mRNA (C) in growing (A) and fully grown (B and C) oocytes. m, micropyle; f, follicle cells; c, chorion.
(D) FISH analysis of pou5f3 mRNA (green) in fully grown oocytes. An inset shows an enlarged view of the boxed region. The chorion is outlined by broken lines. DNA is shown in blue.
(E–L) FISH analyses of pou5f3 (green) and cyclin B1 (red) mRNAs in fully grown immature oocytes (E–H) and mature oocytes (I–L). DNA is shown in blue. H and L show enlarged views of the boxed regions in G and K, respectively.
(M) Numbers of pou5f3 (left) and cyclin B1 (right) RNA granules per 100 μm2 in individual immature (Im) and mature (M) oocytes were counted (means ± standard deviations). ∗∗∗∗p < 0.0001 (Student’s t test). The numbers in parentheses indicate the total numbers of oocytes analyzed. Similar results were obtained from three independent experiments.
(N) PAT assay for pou5f3 and cyclin B1 mRNAs in immature (Im) and mature (M) oocytes. Similar results were obtained from three independent experiments.
(O) FISH analysis of pou5f3 (green) and mos (red) mRNAs in fully grown immature oocytes. Bars, (A–C, E–G, and I–K) 50 μm and (D, H, L, and O) 10 μm.