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Fig. 4
Grm8a is partly expressed in gad1a positive neurons in the developing zebrafish brain. (A) Whole-mount RNA in situ hybridization of grm8a expression at 36 hpf. (B) Magnified picture of the region boxed in (A). Scale bar, 200 μm (overview) and 100 μm (magnification). (C–T) Double labeling of grm8a (blue) and gad1a (red) expression in 36 hpf old embryos using two-color RNA in situ hybridization. Lateral (C,D) and dorsal (F,G) overview of embryonic CNS with anterior to the left. (E,H) Magnifications of boxed areas in (C,G). Dashed lines in (C,D) indicate cutting sites for cross sections shown in (I–R). (M–P,S,T) Magnifications of boxed areas in (I–L,Q,R), respectively. Arrowheads indicate sites of co-localization. Abbreviations are listed in Table 1. Scale bars: 100 μm (overview), 50 μm (magnified images).