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Fig. 5
Efficacy of combined drug treatment in the Rd12 mouse eyes (Mt) compared to wildtype eyes (Wt). (A) Quantitation of the number of TUNEL-positive nuclei in the ONL or INL relative to the total number of nuclei in each layer. Drugs were given either postnatally (Post At+AM), or prenatally and then postnatally (Pre AT+AM). *P < 0.01; **P < 0.001; N = 5. (B) Relative expression of Rpe65 assessed by qRT-PCR. Data plotted as mean ± SEM. Significance comparing treatment groups with wildtype levels determined by one-way ANOVA with Tukey post hoc tests (*P = 0.94, N = 6). (C) Quantitation of Rpe65 protein in retinal/RPE extracts. Data plotted as mean ± SEM. Significance comparing treatment groups with wildtype levels determined by one-way ANOVA with Tukey post hoc tests (*P = 0.78, N = 6). (D) Immunohistochemical localization of Rpe65 protein (green) in RPE cells in different treatment groups. Sections were counterstained with DAPI. Size bar in all panels = 25 μm.