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Fig. 1
Mortality curve and experimental workflow chart. (A) Larval zebrafish were exposed to different concentrations of EUMF (100, 200, 300, 400, 500, 600, 700, 800, and 1,600 μg/mL) from 3 to 6 dpf. The mortality was recorded within each group at 3, 4, 5, and 6 dpf. Dead larvae were judged using missing heartbeats. (B) Larvae at 3 dpf were co-exposed to AlCl3 and three different concentrations of EUMF from 3 to 6 dpf. At 6 dpf the zebrafish were subjected to a behavioral test. In addition, we also evaluated the AchE activity, Aβ deposition, and apoptosis in the brain and performed RT-qPCR.
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Full text @ Front. Mol. Neurosci.