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Figure Caption
Fig. S1 Letm1 has a diel rhythm peaking at ZT23.
(A, B, C, D) Immunoblots using 6 and 7 day post fertilization zebrafish protein lysates from WT and letm1−/− fish probed with antibodies against Letm1, Tom20, and β-actin, using additional biological replicates (BR). Immunoblots in (A) were displayed in the main Fig 2I, as representative blots. The obvious irregularity in single and double bands and unstable protein levels of Tom20 disqualified it for protein normalization, though densitometry analysis of Tom20 relatively to β-actin did not indicate significant changes.
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