Image
|
|
Figure Caption
Fig. 2
Figure 2. HTR2A antagonist AT1015 inhibits the melanogenesis promotion effect of 5-HT in B16F10 cells and zebrafish embryos: (a) Masson–Fonta staining of B16F10 cells to show the melanocytes morphology and melanin content with the treatment of 5-HT (100 μM), HTR2A antagonist AT1015 (3 μM), and cotreatment. Scale bar, 50 μm; (b,c) relative tyrosinase activity (b) and melanin content (c) in B16F10 cells treated with 5-HT (100 μM) and different concentrations of AT1015 (0.1, 0.3, 1, and 3 μM) as compared with the control (n = 6). The photos of melanin precipitation in the tube are at the top of the diagram (c); (d) Western blot shows the protein expression of melanin synthesis, MITF, and TYR in B16F10 cells treated with 5-HT (100 μM) or cotreated with AT1015 (3 μM) or ketanserin (30 μM); (e) the morphology of melanocytes in zebrafish embryos at 60 hpf. The left column shows the whole view of zebrafish embryos (lateral view). The right three columns show the amplification photos of head (lateral view), trunk (lateral view), and head (dorsal view). Scale bar, 100 μm. * (p < 0.05) and ** (p < 0.01) compared to control group; # (p < 0.05) and ## (p < 0.01) compared to 5-HT group.
Figure Data
Acknowledgments
This image is the copyrighted work of the attributed author or publisher, and
ZFIN has permission only to display this image to its users.
Additional permissions should be obtained from the applicable author or publisher of the image.
Full text @ Int. J. Mol. Sci.