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Fig. 3
Figure 3. Lymphatic development is attenuated in murine Gprc5b−/− embryos. (A) RNAscope performed on jugular section of Gprc5b−/− embryo, confirming absence of mRNA expression (compare to Figure 1F): Gprc5b probe (magenta), LYVE-1 lymphatic marker (green), Hoechst cell nuclei (blue). (B) E14.5 Gprc5b+/+ littermate control embryo. (C) E14.5 Gprc5b−/− embryos show graded levels of edema (yellow asterisks) and hemorrhagic plaques (blue arrows). Compared to normal Gprc5b+/+ littermate controls (D), representative H&E images showing subcutaneous edema in the back skin (upper panels, yellow asterisks) and blood-filled lymphatic vessels (lower panels, blue arrows) in Gprc5b−/− embryos with moderate to severe edema (E,F). CA, carotid artery; iJV, internal jugular vein; eJV, external jugular vein, JLS, jugular lymph sac.
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Full text @ Int. J. Mol. Sci.