ZFIN Image: Xu et al., 2022, Figure 1

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Figure Caption

Figure 1

Knockout of nur77 in zebrafish by Crispr/Cas9. (A) Schematic representations of Crispr-Cas9 targets and mutant alleles. nur77 consists of 6 exons (filled box). The sgRNA target exon 1, and the sequences of the target region were aligned to the selected allele. The target site was in red font, and the PAM site was in blue font. The obtained nur77^−/− zebrafish mutant lacked 13 bp bases, causing protein translation to stop prematurely. (B) The validation of the expression level of nur77 by qRT-PCR analysis (n = 3); ^*p < 0.05 by t-test. (C) The morphology of WT and nur77^−/− in developmental stages of 12, 24, 48, 72, and 144 hpf. Scale bars indicate 750 μm.

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Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Front Endocrinol (Lausanne)