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Fig. 7
Epistasis experiments show that npas4l and etv2 are downstream of Bmp signalling. (A) In situ hybridisation for endocard:egfp expression in wild type and etv2 mutant with and without the hsp70l:bmp2b transgene. etv2 mutants have reduced endocard:egfp expression, irrespective of Bmp overexpression. (B) Quantification of the staining area shows Bmp overexpression increased the endocard:egfp expression domain whereas endocard:egfp expression is lost in etv2 mutants, regardless of Bmp overexpression. (C-F) Reciprocal experiments involving in situ hybridisation for endocard:egfp expression in wild-type (transgenic negative siblings) or Tg(hsp70l:nog3) embryos injected with 30 pg of etv2 (C) or 10 pg of npas4l (E) mRNA. Quantification of staining area shows that both etv2 (D) and npas4l (F) mRNA overexpression can restore endocard:egfp expression upon Bmp inhibition. Scale bars: 100 μm. Data are mean±s.d. P-values are indicated in graphs. **P<0.01 (one-way ANOVA). ns, not significant.