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Figure 5
Figure 5. Cellular localization of Gfrα1a in zebrafish testis. (A–E) Immunofluorescence for Gfrα1a (green—A; red—B–E) in testis sections of sexually mature zebrafish. The spermatogonial generations, including type A undifferentiated spermatogonia (Aund), type A differentiated spermatogonia (Adiff) and type B spermatogonia (SPG B), were immunoreactive to Gfra1a, although staining patterns among them varied according to developmental stage. The signal was not found in spermatocytes (SPCs), spermatids (SPTs) and spermatozoa (SPZ). Note that Sertoli cells (SCs) contacting germ cells at different stages of development were also immunoreactive to Gfrα1a. Cell nuclei were counterstained with propidium iodide (A) or Hoechst (B–E). Scale bars: 15 µm. (F) Gfrα1a (approximately 52 kDa (kilodaltons)) immunoblots of whole testes with (+) or without (−) preadsorbed antibodies, confirming the presence of the protein in the zebrafish testes and antibody specificity.
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