Figure 9

Figure 9. Predicted regulatory sequences upstream of human GDNF, mouse Gdnf and zebrafish gdnfa. (A) The upstream region (2000 bp) of human GDNF contains three different sequences of cAMP response elements (CRE), four E-box sequences, three N-box sequences and one Nf-kB binding site. The upstream region (2000 bp) of mouse Gdnf contains an N-box/E-box-rich region at bp −1300 to −1900 and additional E-boxes downstream, one androgen receptor binding site (AR), two Nf-kB binding sites and three different sequences of CRE close to the TSS (transcriptional start site). The upstream region (2000 bp) of zebrafish gdnfa contains four E-box sequences and one N-box sequence, two AR half sequences and only one CRE close to a GC-box and the TSS. TSS is the transcription start site (position +1). (B) Sequences of putative binding sites upstream of zebrafish gdnfa. In the open orange box is shown the TATA-box sequence, in the open gray box the GC-box, in the dark green box the CRE, in the pink box the E-box, in the blue box the N-box, and in the filled orange box the AR half binding site.