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Figure 5
Figure 5. Cellular components of defect at 300 dpi: (i) (A) Uninjured retina (0 dpi) showing typical staining of cones with zpr-1 antibody. (B) Absence of restoration of cone photoreceptors within the injured retina at 300 dpi. (C) Uninjured retina (0 dpi) showing typical staining of rods with zpr-3 antibody. (D) Incomplete restoration and disorganization of rod photoreceptors within the lesion at 300 dpi. (E) Uninjured retina (0 dpi) showing typical staining of horizontal cells with zns-2 antibody. (F) Absence of horizontal cells within the lesion at 300 dpi. (G) Uninjured retina (0 dpi) showing typical staining of MG with zrf-1 antibody. ILM and OLM labelled for reference. (H) Partial restoration of MG within the residual lesion at 300 dpi. All sections were counterstained with DAPI. zpr-1, zpr-3, zns-2, zrf-1 markers—green; Nuclei, DAPI—blue. Retinal layers labelled in control panel A for reference; RPE—retinal pigment epithelium, OLM—outer limiting membrane; ONL—outer nuclear layer, OPL—outer plexiform layer, INL—inner nuclear layer, IPL—inner plexiform layer, GCL—ganglion cell layer; ILM—inner limiting membrane. Images (A,C) at 40×, rest of images at 20×. Scale bars represent 100 µm. (ii) Relative percentage of cell markers (zpr-1, zpr-3, zns-2, zrf-1) at 0–300 dpi (n = 2), * p value < 0.05; *** p value <0.005.
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