ZFIN Image: Huang et al., 2022, Figure 5

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Figure Caption

Figure 5

Other NELF subunits play similar roles in primitive granulocytic development. (A) Q-RT-PCR analysis of nelfa and nelfe expression at different stages. The relative gene expression was normalized to β-actin. (B) WISH for scl, pu.1, mpx, mfap4, or gata1 in WT, nelfa, or nelfe morphants at 22 hpf. Yellow arrowheads and red arrowheads, respectively, indicate ALPM and PLPM. (C–G) Quantification of WISH results in (B) (n = 30–50 embryos per group). All results are presented as the mean ± SD from three independent experiments t test, * for p < 0.05, ** for p < 0.01, *** for p < 0.001, **** for p < 0.0001). “+++” and “+” in (C–G) respectively represent strong staining and weak staining.

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Acknowledgments

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