ZFIN Image: Wiggin et al., 2022, Figure 8.

Image

Figure Caption

Figure 8.

V3-IN laser ablation reduces the proportion of MNs that are active during fictive swimming. MNs in Tg(vglut2a:DsRed)^nns9 larvae were loaded with Calcium Green-1 AM dye and assigned ROIs during recording. Synchronous MN fluorescence and fictive swimming recordings were compared between Control, V3-IN Ablated, and Sham ablated preparations. A, B, Pseudocolored Calcium Green-1 AM fluorescence panels correspond to numbered time points in aligned ΔF/F (each gray line represents a MN) and PN traces in a Control preparation (A) and a V3-IN Ablated preparation (B). Color indicates fluorescence intensity. C, D, Cumulative probability distributions (C) and percent of MNs active (D) during fictive swimming. Asterisks indicate significant differences at p < than 0.01. Error bars represent SD.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ eNeuro