ZFIN Image: Laghi et al., 2022, Figure 11

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Figure Caption

Figure 11

SARS-CoV-2 does not replicate on EPC cells transfected with human ACE2. (A, B) Confocal microscopic images of EPC cells cultures 3 days after transfection. (A) Assessment of transfection efficiency at low magnification. (B) Demonstration of hACE2 expression at the membrane of cells transfected with both plasmids, high magnification, merge images of GFP, hACE2, and nuclei labels. (C, D) qRT-PCR measurement of N copies in cells. (C) Measurement at 2 days postexposure with virulent SARS-CoV-2, comparison of GFP and GFP+hACE2 overexpression. (D) Decline of RNA levels from day 0 to day 2 postexposure, and comparison of heat-inactivated and virulent virus.

Acknowledgments

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