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Figure 3
Macrophage infiltration is increased in HFD, Li-PIC3, and Li-PIC3+HFD larvae. (A–D) 7 dpf larvae labeled with GFP immunostaining to reveal macrophages and either a 2CLIP transgene (A,B) or immunolabeled with Prox1 antibody (C,D) to reveal hepatocytes. Infiltrating macrophages are indicated by white arrows. (E) Quantification of macrophages within the liver boundary reveals a statistically significant increase with HFD and Li-PIC3+HFD treatments. Statistical analysis by one-way ANOVA, ns-non significant, ** p < 0.01, *** p < 0.001.
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