Fig 9

Fig 9

A1: The expression level (given in normalized CPM) of the EE hormones was obtained from the RNA-seq data of Tg(pax6b:GFP⁺) EEC isolated by FACS from wt or neurod1^ΔUCE homozygous mutant larvae at 4 dpf. The values are the expression mean of 6 wt and 4 neurod1^ΔUCE samples. Genes underlined in grey are not significantly regulated (FDR > 0.1). S3 Table provides the expression level of all differentially expressed genes (values for each sample, means, log2 FC and FDR). A2. Log2 fold change (FC) and FDR in the hormone cell number observed in wt compared to neurod1^ΔUCE homozygous mutant embryos as quantified on Fig 9B. A3. Log2 FC and FDR in the hormone cell number observed in control siblings compared to neurod1^-/- homozygous mutant embryos as shown on Fig 11C. Grey zones indicate non-significant change: FDR >0.05). B: Quantification of the number of EECs detected either by FISH performed at 96hpf with mlnl, adcyap1a, insl5a, gcga, galn and pyyb probes or by immunodetection of Penka cells on wt or neurod1^ΔUCE homozygous mutant embryos. Quantification was done by counting the cells using the Imaris software after confocal scanning except for mlnl were the cells were directly counted under a fluorescent stereomicroscope. The mean is indicated by a dashed line and the S.E by solid lines. Asterisks indicate that the difference between the cell number in wt controls and neurod1^ΔUCE mutants is statistically significant using the Mann-Whitney U-test (***: P <0.001; **: P <0.01; *: P <0.05, ns: P >0.05).