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Fig. 3 IFN-υ expressing plasmid and different morpholinos were co-injected into zebrafish embryos (n = 500) at one-cell stage and 48 h later the expression of ISGs was detected by quantitative RT-PCR. The expression of the selected genes was normalized against gapdh and fold changes were calculated relative to control group which was co-injected in embryos (n = 150) with empty plasmid (i.e., vector) and standard (std) control morpholinos.
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Full text @ Nat. Commun.