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Figure 2
Figure 2. The expression of opn1mw2 is reduced in mct8-deficient larvae. (A–D) Ventral (A,C) and lateral (B,D) views of the spatial expression pattern of opn1mw2 mRNA in 3 dpf WT (A,B) and mct8 −/− (C,D) larvae. Scale bar = 300 μm. (B–E) The relative levels of opn1mw2 mRNA were determined using qRT-PCR. (E) Expression levels in 3, 4, and 6 dpf mct8−/− and WT sibling larvae (n = 5 batches of larvae for each group). (F) Expression levels in 6 dpf mct8−25bp−/− larvae (n = 5 batches of larvae for each group). (G) Expression levels in 3 dpf mct8−/−, WT siblings, and mct8 mRNA-injected mct8−/− embryos (n = 5 batches of larvae for each group). (H) The expression levels of opn1mw2 in WT larvae treated with the TH inhibitor methimazole (MMI) (n = 5 batches of larvae for each group). Relative gene expression was normalized against actb in all assays. Statistical significance determined by t-test: two-sample. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001.
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