ZFIN Image: Gao et al., 2022, Fig. 4

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Figure Caption

Fig. 4

Simultaneous loss of Pou5f3 and Sox19b leads to increase in dorsal gene expression and premature activation of differentiation factors.

a Heatmap for 1062 zygotic transcripts, upregulated in the mutants compared to the wild type. The upregulated transcripts were grouped as indicated in the left (groups I, J, K), and sorted by ascending switch time and switch p-value in the respective mutant. b The groups I, J, K were tested for enrichment in Gene Ontology terms using DAVID. Top four categories per group/example genes are shown. Note that the group I upregulated in MZsox19bspg is enriched for the regulators of transcription and for developmental genes. c Mean zygotic transcription profiles for the groups which are upregulated in the mutants compared to the wild type (I, J, K), relative to 2.5 hpf. Note the early upregulation of the transcripts over the WT in MZsox19bspg (group I) and MZspg (group J), but not in MZsox19b (K), where the transcripts are mostly upregulated at 5 hpf (red arrow). MZsox = MZsox19b. n(wt) = 4, n(MZspg) = 2, n(MZsox19b) = 2, n(double) = 2, where n is a number biologically independent experiments. Data are presented as mean values ± SEM. Source data for a, c are provided as a Source Data file. Source data for a, b are provided as Dataset ^S2.

Acknowledgments

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