ZFIN Image: Gao et al., 2022, Fig. 2

Image

Figure Caption

Fig. 2

Maternal Pou5f3 and Sox19b safeguard correct dorso-ventral patterning.

a Comparison of the single mutants, double mutant and wild-type embryos. 10 hpf: MZsox19bspg mutants are arrested in gastrulation similarly to MZspg. Arrow shows abnormally enlarged shield in MZsox19bspg. b, c Double maternal mutants Msox19bspg are dorsalized. b In situ hybridization for dorsal markers noggin1 and Chordin, lateral views, dorsal to the right. Note the circumferential expansion in the Msox19bspg. c Somites (arrowheads) form on the dorsal side in WT, but spread over the Msox19bspg embryo. Dorsal up, anterior to the left. d Normal development of Msox19bspg mutants can be rescued by reducing Chordin, but not Noggin1 levels. The wild-type or Msox19bspg embryos were injected with the indicated morpholinos or non-injected. The numbers show the ratio of embryos with indicated phenotype/ all embryos alive at 22 hpf. The arrows show abnormally expanded blood progenitor cells in the ventralized wild-type embryos. Anterior to the left, dorsal up. e, f Combinatorial (e) and distinct (f) functions of Pou5f3 and SoxB1. e Maternal Sox19b and Pou5f3 safeguard correct D/V patterning. f Pou5f3 is critical for epiboly and gastrulation, redundant action of zygotic SoxB1 (Sox19a, Sox19b, Sox2, and Sox3) is critical for organogenesis. Scale bars in a, b, c: 100 µm, in d: 200 µm.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Nat. Commun.