ZFIN Image: Baronio et al., 2021, Fig. 3.

Image

Figure Caption

Fig. 3. The dopaminergic and histaminergic systems of mao mutants. (A,B) Ventral views of whole-mount 10 dpf mao^+/+ (A) and mao^−/− (B) larval brains, anterior to the left, processed for vmat2 RNA ISH. n=4 for each genotype. (C) Bar charts showing results from RT-qPCR analysis of vmat2 in larvae of the indicated genotype at 10 dpf. n=4 for each genotype. (D-F) Ventral views of whole-mount 10 dpf mao^+/+ (D), mao^+/− (E) and mao^−/− (F) larval brains, anterior to the left, processed for tyrosine hydroxylase 1 (Th1) immunostaining. n=5 for each genotype. (G-I) Ventral views of whole-mount 10 dpf mao^+/+ (G), mao^+/− (H) and mao^−/− (I) larval brains, anterior to the left, processed for histamine (HA) immunostaining. n=5 for each genotype. (J,K) Bar charts showing results from Th1- and histamine-immunoreactive cell counting in larval brains of the indicated genotype at 10 dpf. (L,M) Bar charts showing results from RT-qPCR analysis of th1 (L) and hdc (M) in larvae of the indicated genotype at 10 dpf. Data are mean±s.e.m. One-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *P<0.05, **P<0.01, ***P<0.001. Scale bars: 75 μm.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.