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Fig. 2. The serotonergic system of mao mutants. (A-C) Ventral views of whole-mount 10 dpf mao+/+ (A), mao+/− (B) and mao−/− (C) larval brains, anterior to the left, processed for serotonin (5-HT) immunostaining. Arrow indicates ectopic cells in mao−/− brain. n=5 for each genotype. (D-F) Ventral views of whole-mount 10 dpf mao+/+ (D), mao+/− (E) and mao−/− (F) larval brains, anterior to the left, processed for tph1a RNA in situ hybridization (ISH). (G-I) Bar charts showing results of 5-HT-immunoreactive cell counting in larval brains [paraventricular organ anterior part (PVOa; G), paraventricular organ intermediate part (PVOi; H) and paraventricular organ posterior part (PVOp; I)] of the indicated genotype at 10 dpf. (J,K) Bar charts showing results of reverse transcriptase-quantitative PCR (RT-qPCR) analysis of tph1a (J) and mao (K) in larvae of the indicated genotype at 10 dpf. Data are mean±s.e.m. One-way ANOVA followed by Tukey's post hoc test was used for statistical analysis. *P<0.05, **P<0.01, ***P<0.001. Scale bars: 75 μm.