Fig. s6

Fig. s6 Expression of synthetic TH activity reporter in skin. (A)The synthetic TH reporter TRE-bglob:EGFP was broadly expressed in skin of euTH, but not hypoTH individuals (n=8 each condition). Weak fluorescence evident along the deeper horizontal myosepta in hypoTH represents TH independent expression of the reporter. (B) Higher magnification images showing reporter expression in superficial epidermis and deeper dermis in original planes of image capture (lower left and right, respectively) and in an orthogonal projection (above). All images were captured in 8.6 SSL larvae just prior to onset of squamation and before scales are overtly visible (Aman et al., 2018; Parichy et al., 2009). Scale bars, 100 μm.