ZFIN Image: Torregrosa-Carrión et al., 2021, Fig. 3.

Image

Figure Caption

Fig. 3.

Trabeculation is unaffected in gpr126^bns341 and gpr126^bns342 zebrafish mutants.

(A and B) RT-qPCR analysis of relative gpr126 and gpr112a mRNA expression (to the housekeeping gene rpl13a) in WT zebrafish and heterozygous and homozygous gpr126^bns341 (A) and gpr126^bns342 (B) zebrafish mutants at 48 hours postfertilization (hpf). Data are means ± SD (n = 1, 2, or 3 pools of three hearts per pool). (C to E) Lateral views of 100 hpf gpr126^+/+ (E), gpr126^{bns341/bns341} (D), and gpr126^{bns342/bns342} (E) zebrafish larvae. Inset panels: High-magnification views of the heart (boxed area). (F to I) Mid-sagittal confocal sections of gpr126^+/+ and gpr126^{bns342/bns342} zebrafish hearts at 80 hpf (F and G) and 96 hpf (H and I) in larvae derived from gpr126^bns342/+;Tg(kdrl:Hsa.HRAS-mCherry);Tg(myl7:EGFP-Hsa.HRAS) incrosses. (J and K) Mid-sagittal confocal sections of gpr126^+/+ and gpr126^{bns341/bns341} zebrafish hearts at 96 hpf in larvae derived from gpr126^bns341/+;Tg(myl7:EGFP-Hsa.HRAS) incrosses. Endocardial and myocardial membranes in (F) to (K) are labeled red and green, respectively. Right panels: High-magnification views of the outer curvature of the ventricular wall corresponding to the boxed areas. Arrowheads indicate emerging trabeculae. Scale bars, 25 μm. bv, bulboventricular valve; v, ventricle.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci Adv