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Figure 1
A schematic diagram of generating gRNA templates. Each template contains the T7 promotor, a gene-specific gRNA target, and a common gRNA scaffold. The template is synthesized by ligating three fragments together (in purple, blue, and yellow color), which are annealed from six chemically synthesised oligonucleotides (1–6). The sequences of the overhangs of these three annealed fragments are shown. White letters in the purple fragment designate the gRNA target sequence.
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