FIGURE 1

hoxb5b is expressed during early NCC development. (A) Schematized embryo illustrating approximate locations of major relevant anatomical features, namely the post-otic domain (POD), eye, yolk, Otic Vesicle (Ov), Hindbrain (Hb), presumptive spinal cord (Sc), foregut mesenchyme (Fg), and Midgut mesenchyme (Mg). (B–D) in situ hybridization demonstrating hoxb5b expression in the posterior Hb, POD, and Fg during the second through third day of development. (E–P) Hybridization Chain Reaction probes against crestin and hoxb5b highlight their overlapping domains in the POD (yellow arrowheads) and along the dorsal length (white arrowheads). Scale bars E,H: 50 μM.