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Fig. 4A (A) The GRP78 expression levels were normalized with GAPDH expression in zebrafish testes (n = 3). (B) Expression profiles of the autophagy-related genes in the testes of zebrafish exposed to MCLR (n = 4). (C) ATG5, ATG12, LC3BⅠ and LC3BⅡ in the testes of zebrafish were examined by western blotting (n = 3). (D) Representative immunofluorescence pictures and quantified data to detect LC3B levels in control and MCLR-treated zebrafish testes. Scale bar: 50 µm. * P < 0.05 significantly different from vehicle control group. #P < 0.05 significantly different between different MCLR groups.
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