ZFIN Image: Hamilton et al., 2022, Fig 5

Image

Figure Caption

Fig 5

Decreased pH is necessary and sufficient for intestinal hyperinflammation.

(A) Quantification of luminal pH in intestinal bulb plotting red + green–blue integer values (see Methods) of CV WT and sox10 mutants after 24 hour exposure to 200 μM proton pump inhibitor omeprazole (Omz) or carbonic anhydrase inhibitor acetazolamide (ACTZ) 20 min after m-Cresol Purple gavage. (B) Quantification of intestinal neutrophil number per distal intestine in CV WT and sox10 mutants after 24 hour exposure to 200 μM Omz or ACTZ. (C) Representative images of WT after ACTZ treatment and (D) sox10 mutant after Omz treatment. In A & B, each dot is a fish, n>10. Boxes represent the first to third quartiles, center bar denotes the median, and whiskers the maximum and minimum of each dataset. * p < 0.05, **p<0.01, ****p<00001. ANOVA followed by Tukey’s post hoc test.

Figure Data

Phenotype details

Acknowledgments

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