ZFIN Image: Karuppan et al., 2021, Figure 8

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Figure Caption

Figure 8

Binding specificity in CNTN–amyloid interactions.A, sequence alignments of mouse CNTN1–6 for a subset of residues at the CNTN–amyloid interface. Identical residues are shown in black. Magenta dots indicate the positions of amino acids that are mutated in mouse CNTN4 in the experiments shown in B–E. B–E, analysis of CNTN4–APP interactions by BLI following mutations at the CNTN4–APP interface. In all experiments, the biotinylated E1 domain of mouse APP or its A126V variant was immobilized onto streptavidin sensors and titrated against varying concentrations of the FN1–FN3 region of mouse CNTN4 proteins. One representative experiment for each experimental series is shown in B–E. F, summary of affinities measured for three biological replicates for each set of proteins. APP, amyloid precursor protein; BLI, biolayer interferometry; CNTN, contactin; FN1, first FN repeat; FN3, third FN repeat.

Acknowledgments

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