Fig. 5
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Fig. 5 The otofB mutation results in altered expression of hair cell–related transcripts. (A–F) Quantification of mRNA by qRT-PCR for myo6b, atoh1a, s100s, pvalb9, ret4rl2a, ret4rl2b in wild-type and otofB larvae at 72, 96, and 120 hpf. (A) Quantification of myo6b mRNA, (B) quantification of Atoh1a mRNA, (C) quantification of s100s mRNA, (D) quantification of pvalb9 mRNA, (E) quantification of rtn4rl2a mRNA, (F) quantification of rtn4rl2b mRNA in wild-type and otofB larvae at 72, 96, and 120 hpf (t test, p < 0.001). N = 4 for both WT and mutant, with each N as a composite of 20–30 larvae. The data presented are representative of three experimental replicates for each time point. Error bars are SEM.