Figure 4

Optic nerve injury (ONI) and brca2 genotype exert variable effects on the development of proliferative and neoplastic lesions in the optic nerve pathway. (a) The majority of zebrafish ocular tumors highly express sox10 regardless of ONI status. White arrows indicate dispersed melanin pigment within tumors. Inset shows tumor cells with positive nuclear sox10 expression (brown chromogen). Asterisk indicates a blood vessel containing nucleated erythrocytes that do not express sox10. (b) In zebrafish that received ONI, most brca2 m/m;tp53 m/m zebrafish developed atypical spindle cells in the choroid rete (CR) on the injured side (ONI side) or bilaterally, in contrast to ocular tumor development. (c) In zebrafish that received ONI, brca2 m/m;tp53m/m zebrafish were more likely to develop ocular lesions (ocular tumor and/or hyperplastic spindle cells in the choroid rete) and these lesions were more likely to be bilateral. HE, hematoxylin and eosin; Ret, retina; RPE, retinal pigmented epithelium; ONI, optic nerve injury; CR, choroid rete. Scale bar = 50 µm.