ZFIN Image: Lu et al., 2022, Figure 7

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Figure Caption

Figure 7

LPS enhanced HCC progression in adult twist1a+/kras+ transgenic zebrafish. Twist1a+/kras+ transgenic zebrafish were treated at 3 mpf with Dox and 4-OHT or with Dox, 4-OHT and LPS exposure. Samples were taken at 4 wpi. (A) Immunofluorescence analysis of mCherry and EGFP-labeled metastatic liver tumors and H&E staining of liver tissues from twist1a+/kras+ and twist1a+/kras+/LPS zebrafish at 4 wpi (white dotted frame: primary and metastatic liver tumors). (B,C) No significant differences in body lengths or weights were found between twist1a+/kras+ and twist1a+/kras+/LPS transgenic zebrafish at 2 and 4 wpi. (D) Kaplan-Meier survival curves show the percentage of survival up to 4 wpi. (E) Histological analysis revealed that twist1a+/kras+ and twist1a+/kras+/LPS transgenic zebrafish developed HCC or metastatic HCC at 4 wpi. Scale bar: 50 or 200 μm. Student’s t-test or one-way ANOVA were used to assess differences between variables: ** p < 0.01.

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Phenotype details

Acknowledgments

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