Figure 5

Abnormal folate metabolism results in changes of zebrafish embryo heart development gene expression. (A) Results of qPCR for hand2, nkx2.5, gata4, bmp4, tbx2b, spp1, hoxb1a, nppa in the embryonic heart of zebrafish in each group. (Student t-test, * p < 0.05, *** p < 0.001, **** p < 0.0001, ns: no statistical difference.) (B) In situ hybridization showed the expression of hand2, gata4, nppa in different groups of 3 dpf zebrafish embryonic hearts. (a–d‘) is the in situ expression of the hand2 gene at different exposure concentrations, (e–h‘) is the in situ expression of the gata4 gene at different exposure concentrations, (i–l‘) is the in situ expression of the nppa gene at different exposure concentrations. A: atrium V: ventricle OFT: outflow tract. n = 3. Scale bars: 200 μm.