ZFIN Image: Han et al., 2021, Figure 4

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Figure 4

Generation of mthfr mutant using the CRISPR/Cas9 system. (A) Design of sgRNA target on exon 2 of zebrafish mthfr gene. The mutation type of code shift selected by orange frame is selected finally. (B) Two short DNA fragments were obtained by T7E1 digestion. In the control group, 437 bp fragment was amplified from wild-type embryos with the same set of primers. (C) The predicted truncation of Mthfr protein. (D) Sequencing map of homozygous zebrafish adults. (E) Bright-field views of mthfr^−/− zebrafish showed Pericardial enlargement, ventricular enlargement and abnormal heart looping. Scale bars: 500 μm. (F) The results of in situ hybridization showed that the expression of mthfr gene in homozygous mutant zebrafish was changed.

Acknowledgments

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