Figure 2

Folate excess and deficiency lead to different degrees of abnormal cardiac physiological function and abnormal metabolism of homocysteine. (A) The phenotypes of zebrafish embryonic heart treated with different concentrations of folic acid and MTX inhibited folate metabolism pathway were demonstrated at 3 dpf. (a–d) is the view under light microscope, (e–h) is cmlc2:EGFP labeled cardiac fluorescence images, (i–l) is cmlc2:EGFP and kdrl:mCherry Co labeled confocal images of the heart. (m–p) is images of a paraffin section of a zebrafish heart after staining by hematoxylin-eosin. Scale bars: 100 μm. (B) Statistics on the influence of different folic acid treatment groups on the looping angle of zebrafish embryo heart. (C) Statistics on the influence of different folic acid treatment groups on the thickness of the ventricular myocardium of zebrafish embryos. (D) High homocysteine content in zebrafish of different groups. (Student t-test, * p < 0.05, ** p < 0.01, **** p < 0.0001, ns: no statistical difference).