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Fig. 2 (A) Illustration depicts concentric anatomical layers of the proximal (small) intestine and associated macrophage populations in zebrafish (A, left) and mouse (A, right). (B) Illustration depicts ex vivo imaging strategy with the muscularis positioned nearest the imaging objective. (C and D) (C) Quantification of mucosa and muscularis-associated mpeg1GFP+ macrophages in several fields of view per animal pooled from n = 3 Tg(mpeg1:GFP) adult ex vivo gut by region-specific z-projection as shown in (D) scale = 50 μm. (E) Representative Nyquist-sampled confocal imaging micrographs (voxel depth 3 μm) highlighting regional diversity of mucosa- or muscularis-associated mpeg1GFP+ fluorescence in the concentric layers of the gut: lamina propria, circular muscle, myenteric plexus, and longitudinal muscle. p value was determined by a two-tailed T test. Scale bars are shown as indicated.