ZFIN Image: Fadul et al., 2021, Fig. 1

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Figure Caption

Fig. 1

KRas^V12 induces formation of epidermal cell masses and basal extrusion at distinct sites.

a 26 hpf wild-type zebrafish embryos expressing krt4:EGFP-CAAX or dt-KRas^V12, masses indicated by black arrowheads and inset. b Mean of cell masses per embryo ± SEM, P < 0.0001 by a Mann–Whitney test, n = embryos. c Map of where cell masses occur, as observed at 28 hpf. d Stills from time-lapse imaging (hh:mm) of periderm:Lifeact-EGFP mosaically expressing EGFP-CAAX or EGFP-dt-KRas^V12, showing hallmarks of apical and basal extrusion, with XZ sections beneath showing constriction at the base or apex, respectively (white arrows delimit ring constricting). e Number of apical and basal extrusions in EGFP-CAAX or dt-KRas^V12 embryos. Data are mean ± SEM, **P < 0.02 by a Mann–Whitney test for apical and basal extrusions pooled together. f Maps indicating where apical and basal extrusions occur in 28-46 hpf embryos and 55–73 hpf embryos, where different colours represent different fish analyzed, n. Source data are provided as a Source Data file.

Acknowledgments

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