Figure 1

Figure 1

Zebrafish cyp20a1 gene map and allele sequences. (A) Gene models. (B) Gel image showing PCR products and amplicon fragments derived from T7E1 mutant survey (F₀). Lower gel shows the positive (heteroduplexed - red arrows) T7E1 signature. (C) Chromatograms from F₁ heterozygous embryos (wh⁶⁰ and wh⁶¹) beginning near the sg1 site. (Note the appearance of double peaks). (D) cDNA sequences for exons 1–3 for WT, wh⁶⁰, wh⁶¹. Note wh⁶⁰ is a 5 bp deletion and 4 bp insertion in exon 2 and wh⁶¹ is a 1 bp insertion in exon 2, and a 7 bp deletion in exon 3. (E) Putative translation of cDNAs.