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Figure 6
Zebrafish BIRC2 accumulates p53. (A) Zebrafish BIRC2 accumulates p53 in the uninfected EPC cells. (B) Zebrafish BIRC2 accumulates p53 in the E. piscicida-infected EPC cells. (C) Zebrafish BIRC2 accumulates p53 in the cytoplasm of infected EPC cells. (D) Zebrafish BIRC2 accumulates p53 in the nucleus of infected EPC cells. For (A, B), EPC cells transfected with p53-GFP, p3×FLAG and/or BIRC2-FLAG were infected with E. piscicida for 1 h at an MOI of 1 or left untreated. At 6 hpi, the cells were washed, lysed, and subjected to Western Blotting with the indicated antibodies. For (C, D), EPC cells transfected with p53-GFP, p3×FLAG and/or BIRC2-FLAG were infected with E. piscicida for 1 h at an MOI of 1. At 6 hpi, the cells were harvested and used for preparation of nuclear and cytoplasmic extracts. Tubulin and HDAC1 were used as loading controls for cytoplasmic and nuclear protein, respectively. The expression ratio for p53 protein was quantified by Quantity One.