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Figure 3
Overexpression of zebrafish BIRC2 inhibits the early and late apoptosis in the case of E. piscicida infection. (A) The effect of zebrafish BIRC2 on the early apoptosis. (B) The effect of zebrafish BIRC2 on the late apoptosis. The EPC cells transfected with p3×FLAG or BIRC2-FLAG were infected with E. piscicida at an MOI of 5, 10 or left untreated. At 6 hpi, samples were collected, then stained with annexin V FITC, and finally analyzed by cytoflex S Flow Cytometer. Data represented the means ± the SEM (n = 3) and were tested for statistical significance using a two-tailed student’s t-test. *p < 0.05; **p < 0.01.