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Figure 2
A schematic diagram for the experimental design for drug treatment and discontinuation. To determine mRNA expression of RAAS genes following one week of chronic antihypertensive drug administration, Group 1 consisted of zebrafish that were treated with 0.2% DMSO (control), aliskiren, olmesartan, captopril, and amlodipine daily for 7 days, then dissected for qRT-PCR analysis. Additionally, Group 2 consisted of zebrafish that were treated identically as Group 1 for 7 days, but then drug administration was discontinued for 7 more days before dissection and qRT-PCR analysis. The extracted organs include the brain, kidney, intestine, gill, liver, and heart. Six biological replicates (3 males and 3 females) were used for each compound (or vehicle) in each group.