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Figure 5
Effect of pre-injected Zetnf-b polyclonal antibody or AG-126 to zebrafish before challenging with Vibrio vulnificus. (A) The effect of pre-injection of a certain dose of Zetnf-b polyclonal antibody on the survival rate before challenging with V. vulnificus, using pre-immune rat serum as control. The survival rate was 73.3% at the dose of 2.5 μl/tail. (B) The effect of pre-injection of a certain dose of AG-126 on the survival rate before challenging with V. vulnificus, using phosphate-buffered saline (PBS) as control. The survival rate was 80% at the dose of 2.5 μg/tail. Zebrafish survival rate was calculated according to the formula. Survival rate %=(Survived zebrafish / Total fish) × 100%. Data are presented as mean ± standard error (SE) from three independent experiments. * Indicates significant difference at P < 0.05.