ZFIN Image: Kenney et al., 2021, Figure 3.

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Figure Caption

Figure 3.

Image registration pipeline.

(A) Raw TO-PRO images from 17 fish were first aligned using linear transformations (LSQ6 and LSQ12) followed by a final nonlinear transformation (right). Deformation grids at each stage are overlaid. Consensus average images at each stage of the pipeline are given below. (B) Raw autofluorescence images (top) acquired at the same time as the TO-PRO images were registered into the same space using the transformations derived from TO-PRO registration (middle). Images were then averaged together to generate a corresponding autofluorescence average in the same anatomical space as the TO-PRO images (bottom).

Acknowledgments

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