ZFIN Image: Kenney et al., 2021, Figure 2.

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Figure Caption

Figure 2.

Imaging of nuclear-stained tissue-cleared samples.

(A) Image of adult zebrafish brain samples before (top) and after (bottom) clearing using iDISCO+. (B) Example TO-PRO-stained images from a single sample acquired in the horizontal plane during light-sheet imaging. (C) Three-dimensional volumes generated from a set of light-sheet images from an individual brain visualized using a maximum intensity projection (left) and exterior volume (right). (D) Coronal (left) and sagittal (right) views of an individual brain generated from a single three-dimensional volume.

Acknowledgments

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