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Figure 2
ASB2 specifically ubiquitylates SMAD9. HEK 293T cells were co-transfected with vectors coding HA-Ub, Myc-tagged E3 (Myc-ASB2, SMURF1 and SMURF2) and Flag-tagged R-SMADs (Flag-SMAD2, 3, 1, 5, 9) as indicated. Flag-immunoprecipitates or lysates were separated with SDS-PAGE and immunoblotted with the indicated antibodies. The cropped gel images were delineated and uncropped gel images are shown in the Supplementary Information.
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