ZFIN Image: Kenney et al., 2021, Figure 4

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Figure Caption

Figure 4

Imaging and registration of antibody stains.

(A) Representative light-sheet images taken in the horizontal plane from individual brains stained with indicated antibodies. (B) Autofluorescence images acquired during antibody staining (top) were registered into the same space as autofluorescence images acquired during TO-PRO staining (bottom). (C) Transformations from autofluorescence registration were applied to antibody images to bring antibody stains into the same anatomical space as the TO-PRO stain. Yellow crosshairs are in the same place on each image. (D) Example of correspondence between TO-PRO and antibody images and how stains can be used to identify the boundaries of specific nuclei (green arrow: locus coeruleus) and white matter tracts by a lack of staining (pink arrowhead: medial longitudinal fascicle).

Acknowledgments

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